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M-MLV Reverse Transcriptase PCR Reagents R1042

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M-MLV Reverse Transcriptase PCR Reagents R1042

Brand Name : GDSBio

Model Number : R1042

Certification : /

Place of Origin : China

MOQ : 1Bag

Price : US $19.9-59.9 / Bag

Payment Terms : L/C, D/A, D/P, T/T, Western Union, MoneyGram

Supply Ability : 100 Bag/Bags Per Day

Delivery Time : 8work days

Packaging Details : small package or bulk distribute or OEM

Cat. No. : R1042

Concentration : 200U/μl

Appearance : colourless

Group : Reverse Transcriptase PCR Reagents

Activity : Pass

Amplification capability : /

Logo Printing : With Logo Printing

Transport Package : Packing

Production Capacity : 100 Bag/Bags Per Day

Storage Conditions : Store at -20°C

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M-MLV Reverse Transcriptase

High quality M-MLV Reverse Transcriptase Rt-PCR R1042

For research use only

Components of the kit

Component R1041 (5,000U) R1042 (10,000U)
M-MLV (200U/μl) 25 μl 50μl
5× First-strand Buffer 100μl 200 μl

Description
The Dongsheng M-MLV Reverse Transcriptase (RT) is a genetically modified M-MLV RT. It differs from the M-MLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a ribonuclease H activity specific to RNA in RNA-DNA hybrids. Dongsheng M-MLV Reverse Transcriptase has significantly lower RNase H activity than Avian Myeloblastosis Virus (AMV) reverse transcriptase.

Source
E.coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus reverse transcriptase.

Applications
Generation of first strand cDNA for use in:
-PCR, see Protocol for First-strand cDNA Synthesis;
-real-time PCR;
-second strand cDNA synthesis;
DNA labeling;
Analysis of RNA by primer extension.

Quality Control
The absence of endodeoxyribonucleases, exodeoxyribonucleases, phosphatases and ribonucleases confirmed by appropriate quality tests.

Functionally tested in first strand cDNA synthesis.

Store Buffer
20m M Tris-HCl (pH 7.5)
200 mM NaCl
0.1 mM EDTA
1 mM DTT
0.01% NP-40
50% glycerol

5xFirst-Strand Buffer
250 mM Tris-HCl (pH 8.3 at 25°C)
375 mM KCl
15 mM MgCl2
50 mM DTT

M-MLV Reverse Transcriptase PCR Reagents R1042M-MLV Reverse Transcriptase PCR Reagents R1042

M-MLV Reverse Transcriptase PCR Reagents R1042
Dongsheng Biotech offers different series of products to help you achieve PCR success. For enzymes, our Taq Polymerase, HS Taq DNA Polymerase, FS Taq DNA Polymerase, Pfu DNA Polymerase and Fusion Pfu DNA Polymerase provide high fidelity, efficient, sensitivity PCR performance. We also have Long Taq DNA Polymerase for long PCR performance.

M-MLV Reverse Transcriptase PCR Reagents R1042


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M-MLV Reverse Transcriptase PCR Reagents

      

Reverse Transcriptase PCR Reagents M-MLV

      

M-MLV reverse transcriptase rt pcr

      
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